Compartmentalization of telomeres through DNA-scaffolded phase separation
نویسندگان
چکیده
Telomeres form unique nuclear compartments that prevent degradation and fusion of chromosome ends by recruiting shelterin proteins excluding DNA damage repair factors. To understand how these dynamic components protect ends, we reconstitute human in vitro show multicomponent liquid condensates with selective biomolecular partitioning on telomeric DNA. TRF1 TRF2 subunits drive phase separation, their N-terminal domains specify interactions vitro.
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 2022
ISSN: ['0006-3495', '1542-0086']
DOI: https://doi.org/10.1016/j.bpj.2021.11.1219